SnapFast™ Plasmids – simplifying biology using building blocks of DNA
Genetic engineering is used in thousands of laboratories around the world. Given its scientific and commercial importance it is remarkable that cloning strategies for many of the popular DNA components are not standardised. The aim of Oxford Genetics was to engineer a DNA plasmid system that could accommodate most of the functional DNA inserts that a researcher might require within a single plasmid. By optimising their starting vector, every component of the system can be removed and exchanged for hundreds of other DNA sections which have been pre-designed and tested by Oxford Genetics. This is the concept of the SnapFast™ system.
Cleaner DNA – All of the constructs from Oxford Genetics have been pre-screened for poor codon usage and conflicting restriction sites. Where possible, all rare codons and restriction sites have been removed to enable efficient expression, and ensure that conflicting restriction sites do not limit the cloning of other SnapFast™ DNA inserts.
The Oxford Genetics product range includes:
Over 900 available plasmids covering the following types:
|• Bacterial Plasmids||• Mammalian Plasmids||• Lentiviral Plasmids|
|• CRISPR Plasmids||• Adenoviral Vectors|
Vector Sets: Need to explore the best method to express your vector, then Oxford Genetics have 35 vector selection kits. The Bacterial 6His Tag Vector Set (Item Code: PP2389) is an example of how these Vector Sets are used.
Bacterial 6His Tag Vector Set Description: This pack enables researchers to compare placing hexahistidine (6His) affinity tags at either the N or C terminus of your gene of interest (inserted into the MCS, under transcriptional control of the OXB20 strong bacterial promoter) with, and also without a TEV (Tobacco Etch Virus) protease cleavage site. The TEV site enables removal of the 6His tag from the protein following purification. Comparing these four configurations should enable you to evaluate how best to express and purify your gene of interest from bacterial cells. We also provide many other functional tags and cleavage sites, if required.