ChromoTek’s unconjugated Nanobodies or VHHs are well characterized and validated for the listed applications. Because the molecular weight of these single-domain antibodies (sdAb) is just about 15 kDa, VHHs are only one tenth the size of a conventional IgG antibody (150 kDa) or less than a third the size of a Fab fragment (50 kDa). In combination with their high chemical and thermal stability plus high affinity, these binding proteins are ideal for multiple types of affinity assays in biochemistry and microscopy, including immunoprecipitation, immunofluorescence, super resolution microscopy, surface plasmon resonance (SPR), biolayer interferometry (BLI), switchSENSE®* technology, etc.
*Measured with switchSENSE® technology from Dynamic Biosensors
In addition ChromoTek offer a range of conventional antibodies. These mouse, rabbit, and rat antibodies are used for the reliable detection of fluorescent proteins, protein-tags, and small epitope tags as well as important epigenetic markers, and RNA-Polymerase II.
The conventional IgG antibodies complement the ChromoTek alpaca single domain antibody products due to a different antigen recognition mode: Typically, Nanobodies tend to bind to conformational 3D epitopes, whereas conventional antibodies mostly bind linear epitopes, which can be found in denatured proteins, e.g. on a Western blot.
Chromobodies – Intracellular Functional Antibodies or Intrabodies
ChromoTek’s Chromobodies® are fluorescent nanoprobes for real-time and live-cell imaging of intracellular endogenous proteins.
A Chromobody is a small intracellular functional antibody. It consists of a VHH/ Nanobody that is genetically fused to a fluorescent protein. Unlike conventional antibodies, Chromobodies visualize endogenous cellular structures and processes in live cells and real-time.
Nano-Secondaries are the next level of secondary antibodies. They are based on monoclonal recombinant VHHs/ Nanobodies. Nano-Secondaries bind to primary antibodies with high affinity and specificity. Our Nano-Secondaries are conjugated to Alexa Fluor® dyes. Currently, Nano-Secondaries against rabbit and mouse IgG are available.
Peptides & Proteins
ChromoTek offers purified proteins as controls and synthetic peptides for competitive elution. Our proteins and peptides are highly pure and ensure reliable and reproducible results.
The Spot-Tag® is a short peptide tag that is recognised by ChromoTek’s Spot VHH, Spot-Label, and Spot-Trap. A Spot-Tag fusion protein can therefore be analyzed in a wide range of applications such as immunoprecipitation, protein purification, Western blot, immunofluorescence microscopy, super resolution microscopy, ELISA etc.