Pricing and Availability:

A$ Prices are on this website, alternatively, please contact us for prices and availability on any of the ChromoTek’s range of products.

 

Nano-Traps are ideal for fast, reliable and efficient one-step immunoprecipitations

Nano-Traps pull down the proteins of interest (POI) and their interacting factors from cell extracts or organelles. Nano-Traps consist of a VHH antibody, also termed a “nanobody”, coupled to an immobilising matrix (agarose beads, magnetic agarose beads or multiwell plates1).

Note 1
: All Nano-Traps are available coupled to agarose beads and in 96-multiwell plate formats. Selected Nano-Traps are also available coupled to magnetic agarose beads.

What are VHH antibodies?

Next to conventional IgG antibodies, which are composed of two heavy chains and two light chains, the immune repertoire of camelids such as dromedaries, llamas and alpacas contains so-called heavy chain only IgGs. These antibodies lack the CH1 domain of the heavy chain and are devoid of any light chain. Therefore, the variable domain of these heavy chain only IgGs, called VHH or Nanobody, represents a stand-alone antigen binding entity and can be produced as a single domain antibody fragment (sdAb).

 

What makes ChromoTek technology special?

For more than a decade, ChromoTek has generated highly specific and high affinity VHH antibodies for various applications. By combining their proprietary immunisation scheme with phage display, ChromoTek are capable to create recombinant sdAbs with tailor-made properties. This technique provides the best of both worlds: Highly specific antibodies that have undergone affinity maturation in vivo, but that are produced recombinantly with known sequence and without any batch-to-batch variations. Typically, affinities are in the picomolar to single-digit nanomolar range. Subsequently, the VHH domains can be further engineered or adapted, depending on the requirements of a specific application.

 

Applications:

  • Immunoprecipitation (IP) / Co-IP
  • Mass spectrometry
  • On-bead enzyme assays
  • ChIP / RIP analysis

Benefits:

  • No heavy and light chains in your downstream gel and mass spectrometry analysis
  • High binding affinity: Dissociation constants down to 1 pM
  • Short incubation times; reliable and consistent results

 

Nano-Traps are available with the following VHH (nanobody) specifities:

• TurboGFP-Trap • mNeonGreen-Trap • MK2-Trap • SNAP/CLIP-tag®-Trap
• Myc-Trap • Halo-Trap • p53 N-term-Trap • Binding Controls
• RFP-Trap® • MBP-Trap • Mdm4/ HdmX-Trap • PARP1-Trap
• GFP-Trap® • GST-Trap • Spot Trap® • p53 C-term-Trap

 

The GFP-Trap® and RFP-Trap®

GFP-Trap®

Description: The ChromoTek GFP-Trap® for one-step immunoprecipitation (IP) of GFP-fusion proteins. GFP-Trap is an anti-GFP VHH coupled to agarose or magnetic agarose beads. The anti-GFP VHH is also known as GFP-Binding-Protein, GFP Nanobody or anti-GFP single domain antibody (anti-GFP sdAb). ChromoTek GFP-Trap is referenced in more than 1,500 scientific articles and the gold standard for IP of GFP.

Specificity: GFP, EGFP, CFP, YFP, BFP and many more GFP derivatives.

RFP-Trap®

Description: RFP-Trap is an anti-RFP VHH coupled to agarose or magnetic agarose beads. RFP-Trap or RFP-beads are an affinity resin for immunoprecipitation and pull-down of many red fluorescent proteins. The RFP-Trap is referenced in about 100 scientific articles.

The anti-RFP VHH is also known as RFP binding protein, RFP Nanobody or anti-RFP single domain antibody (anti-RFP sdAb) fragment.

Specificity: mCherry, mRFP, mRFPruby, mPlum, tagRFP, mKate2 and many more RFP derivatives.

Advantages:

  • A robust and versatile tool for biochemical analyses of GFP fusion proteins or RFP fusion proteins
  • Short incubation times (5-30 min)
  • Quantitative isolation of fusion proteins and transiently bound factors from various cell extracts and organelles (from all organisms)
  • No non-specific binding or contamination by heavy and light chains of conventional antibodies
  • Functionality for Chromatin Immunoprecipitation (ChIP)

Spot-Tag & Spot-Nanobody

One for All: Small Affinity-Tag & Nanobody for Multiple Capture & Detection Applications. The ChromoTek Spot-System is the first peptide-tag specific Nanobody for universal capture & detection applications. It comprises Spot-Tag®, an inert 12 amino acid peptide-tag (PDRVRAVSHWSS), and the universal, rugged Spot-Nanobody that specifically binds to Spot-tagged proteins with high affinity.

 

Nano-Boosters & Nano-Labels

ChromoTek’s Nano-Boosters & Nano-Labels are fluorescent probes. They consist of very small recombinant antibody domains, also called Nanobodies or VHHs. These single-domain alpaca antibody fragments are covalently coupled to fluorescent dyes.

  • The Nano-Boosters GFP- and RFP-Boosters enhance the signal of fluorescent proteins
  • The Nano-Labels Vimentin- and Histone-Label fluorescently label endogenous cellular proteins
  • The Spot-Label fluorescently labels the Spot-Tag, a 12-aa peptide tag (PDRVRAVSHWSS)

Applications:

Immunofluorescence (IF): immunocytochemistry (ICC) and immunohistochemistry (IHC). Cleared tissue, organ, and animal (DISCO) staining for light-sheet microscopy imaging. Super-resolution microscopy (SRM).

ChromoTek Product Brochures

Click on the brochure image below to open and download the selected product brochure:

GFP-Trap RFP-Trap Nano-Boosters Spot-Trap, Spot-Tag

 

Other ChromoTek Products

ChromoTek’s unconjugated Nanobodies or VHHs are well characterized and validated for the listed applications. Because the molecular weight of these single-domain antibodies (sdAb) is just about 15 kDa, VHHs are only one tenth the size of a conventional IgG antibody (150 kDa) or less than a third the size of a Fab fragment (50 kDa). In combination with their high chemical and thermal stability plus high affinity, these binding proteins are ideal for multiple types of affinity assays in biochemistry and microscopy, including immunoprecipitation, immunofluorescence, super resolution microscopy, surface plasmon resonance (SPR), biolayer interferometry (BLI), switchSENSE®* technology, etc.

*Measured with switchSENSE® technology from Dynamic Biosensors

In addition ChromoTek offer a range of conventional antibodies. These mouse, rabbit, and rat antibodies are used for the reliable detection of fluorescent proteins, protein-tags, and small epitope tags as well as important epigenetic markers, and RNA-Polymerase II.

The conventional IgG antibodies complement the ChromoTek alpaca single domain antibody products due to a different antigen recognition mode: Typically, Nanobodies tend to bind to conformational 3D epitopes, whereas conventional antibodies mostly bind linear epitopes, which can be found in denatured proteins, e.g. on a Western blot.

Chromobodies – Intracellular Functional Antibodies or Intrabodies

ChromoTek’s Chromobodies® are fluorescent nanoprobes for real-time and live-cell imaging of intracellular endogenous proteins.
A Chromobody is a small intracellular functional antibody. It consists of a VHH/ Nanobody that is genetically fused to a fluorescent protein. Unlike conventional antibodies, Chromobodies visualize endogenous cellular structures and processes in live cells and real-time.

Nano-Secondaries

Nano-Secondaries are a novel class of secondary antibodies for higher resolution & better imaging. They consist of Nanobodies that bind their antigen with high affinity & specificity and are conjugated to Alexa Fluor® dyes.

Peptides & Proteins

ChromoTek offers purified proteins as controls and synthetic peptides for competitive elution. Our proteins and peptides are highly pure and ensure reliable and reproducible results.

Spot-Tag Vectors

The Spot-Tag® is a short peptide tag that is recognised by ChromoTek’s Spot VHH, Spot-Label, and Spot-Trap. A Spot-Tag fusion protein can therefore be analyzed in a wide range of applications such as immunoprecipitation, protein purification, Western blot, immunofluorescence microscopy, super resolution microscopy, ELISA etc.