Price and Availability:

Please contact us for prices and availability on any of the 4basebio range of products.

 

High performance genomics products

TruePrime® Technology

TruePrime® technology is based on the combination of the recently discovered DNA primase TthPrimPol1 and the extremely processive and high fidelity Phi29 DNA polymerase. TthPrimPol is a thermostable member of the recently discovered PrimPol family of enzymes. PrimPols are endowed with primase and polymerase activities. The main novelty of these enzymes is their capacity to synthesize DNA primers. This feature allowed 4basebio to develop TruePrime®, a new multiple displacement amplification (MDA) technology based on the combination of TthPrimPol and Phi29 DNA polymerase. As a result, TruePrime® users have a distinct advantage, as TruePrime® does not accept non-denatured DNA strands as templates as readily as random primed MDA reactions. This quality means that any contamination introduced after the denaturation step will not have a strong influence on the composition of the reaction out.

Available Products:

  • TruePrime® Single Cell Whole Genome Amplification Kit
  • TruePrime® Whole Genome Amplification Kit
  • TruePrime® Apoptotic Cell Free DNA Amplification Kit
  • TruePrime® Necrotic Cell Free / Exosomal DNA Amplification Kit
  • TruePrime® Rolling Circle Amplification Kit

CovCheck™ Genome Coverage WGA QC Kits

CovCheck™ Genome Coverage WGA QC Singleplex Kit provides a ready to use set of endpoint PCR primers in a convenient 96 well plate format, complete with optimized PCR reagents including a premium hot start Taq polymerase. The plate includes four identical sets of 24 different primer pairs that will amplify small portions from each human chromosome, allowing the coverage analysis of four independent single cell whole genome amplifications simultaneously. Easily check results with an agarose gel or any other DNA quantification technique.

CovCheck™ Genome Coverage WGA QC Multiplex Kit provides a ready to use set of endpoint PCR primers in a convenient 96 well plate format, complete with optimized PCR reagents including a premium hot start Taq polymerase. The plate includes 16 identical sets of 24 different primer pairs multiplexed in six reations, that will amplify small portions from each human chromosome, allowing the coverage analysis of 16 independent single cell whole genome amplifications simultaneously.

qPCR CovCheck™ Genome Coverage WGA QC Singleplex Kit provides a ready to use set of PCR primers in a convenient 96 well plate format, paired with optimized qPCR reagents. The plate includes four identical sets of 24 different primer pairs that will amplify small portions from each human chromosome, whilst allowing the real time coverage analysis of four independent single cell whole genome amplifications. The optimized qPCR reagents (i.e. qPCR CovCheckTM Genome Coverage WGA QC Singleplex Master Mix: Hot Start Taq DNA polymerase, SYBR Green I, reaction buffer and dNTPs) mean that the qPCR CovCheckTM Genome Coverage WGA QC Singleplex Kit is an easy and convenient approach to getting the best from your single cell experiments, by enabling visualization of specific targets whilst minimising costs.

DNA Polymerases

MagniPhi® Phi29 DNA pol wild type is the wild type Phi29 DNA polymerase currently in use in the TruePrime® DNA amplification kits as supplied by 4basebio. The highly processive capability of the enzyme (up to more than 70kb per binding event) paired with a strong strand displacement capacity makes MagniPhi® Phi29 DNA pol wild type the perfect choice for isothermal DNA amplification. The 3’ –> 5’ proofreading exonuclease activity of the MagniPhi®Phi29 DNA pol wild type means that MagniPhi® Phi29 DNA pol wild type has a very high fidelity of synthesis, resulting in up to 100 times less errors than using Taq polymerase, while the amplification reaction yields more Whole Genome Amplification (WGA) product compared to PCR based techniques. These distinctive features make this enzyme the perfect choice for isothermal DNA amplification or any application requiring a high fidelity DNA polymerase with strong strand displacement capacity.

QualiPhi® Phi29 DNA pol chimera is a novel, highly processive chimeric form of Phi29 DNA polymerase, expertly engineered for enhanced sensitivity and efficiency (de Vega et al., 2010).As the chimeric version of the wild type Phi29 DNA polymerase, QualiPhi® Phi29 DNA pol chimera has preserved the high processivity – strong strand displacement capacity and the excellent 3’ –> 5’ proofreading exonuclease activity resulting in very high synthesis fidelity – of the wild type Phi29 DNA polymerase. QualiPhi® Phi29 DNA pol chimera has been engineered to dramatically increase its sensitivity, thus enabling it to more efficiently amplify DNA from low input concentrations. With enhancements furthermore resulting in yields far exceeding wild type Phi29 DNA polymerases, QualiPhi® Phi29 DNA pol chimera is a premium polymerase giving researchers the opportunity to combine primers with a specialist polymerase enhanced for work with low concentration template DNA.

SunScript® Reverse Transcriptase

SunScript® ReverseTranscriptase Kit is a novel, highly thermostable reverse transcriptase engineered from the well characterized HIV-1 RT. It allows reaction temperatures up to 85°C, has RNA and DNA dependent polymerase activity and offers improved efficiency of cDNA synthesis. The elimination of RNaseH activity provides this enzyme with the best performance to allow high yields of full length cDNA synthesis on long RNA molecules with complex secondary structures.

SunScript®One Step RT-PCR Kit is an easy and reliable system designed for fast, specific and sensitive endpoint RT-PCR reactions. The kit contains all the components needed to perform both reverse transcription and PCR amplification in the same single tube by using gene specific primers, in a “one step reaction”. This minimizes contamination and constitutes a convenient system to process multiple samples.

SunScript® One Step RT-qPCR Kit is an easy and reliable system designed for fast RT-qPCR (real time) reactions based on detection of double stranded DNA binding dyes. One key advantage of the kit is the elevated temperature for performing the RT step thus allowing higher specificity of the reaction, and a higher likelihood in obtaining reaction products for difficult targets. Moreover, the excellent kinetic performance of the SunScript® Reverse Transcriptase allows for speed optimization of the protocol.