Product Code: OG390
Quantity Provided: 5µg
Size (bp): 5452
Bacterial Antibiotic Selection: Kanamycin
Origin and Compatibility: pUC high copy, derived from pBR322
Copy Number: 500-700 copies per cell
Promoter: Cytomegalovirus (CMV) immediate early promoter and the mouse phosphoglycerate kinase promoter (PGK)
Purpose: This plasmid contains two promoters that terminate transcription at the same poly-adenylation signal, allowing the expression of two genes from one expression cassette, where the second gene is the daGFP fluorescent reporter gene. The second promoter (PGK) is approximately 10-fold weaker than the upstream CMV promoter in most commonly used cell lines.
daGFP: This vector encodes a green fluorescent protein called daGFP. This protein can be used in the same way as normal GFP using argon laser based or UV based excitation apparatus to allow the detection of fluorescence. The protein has a peak excitation of 510nm and a peak emission of 521nm. The gene encoding the protein is sold free of intellectual property restrictions.
Quality Validation: This vector has been demonstrated to express reporter genes to high levels under the CMV promoter. The start codon of these reporters was positioned within the NcoI site and the stop codon was positioned within the XbaI site. This plasmid also demonstrates intermediate levels of expression of daGFP from the PGK promoter. Expression has been validated in 293 cells.
Quality Sequencing Analysis: This vector has been fully sequenced using primers F1-F10. The sequences and details of these primers are available through Oxford Genetics website.
Intellectual Property Status: According to the Oxford Genetics IP-friendly policy, this plasmid is sold free of reach-through rights on any derivatives you may create.