Vascular Biology Feature
E-mail Print
Vascular Biology Feature - Plasminogen Activators tPA and uPA
Go to Oxford Biomedical Research website
The activation of plasminogen is required for blood clot degradation, inflammation reduction and tumor metastasis. These processes are facilitated by the activators, tissue-type Plasminogen Activator (tPA) and urokinase-type Plasminogen Activator (uPA).

Oxford Biomedical Research has developed a range of assays, antibodies and related products for researchers working in the area of vascular biology with an interest in understanding the role of tPA and uPA in biological systems.
Literature and Technical Requests
Technical Literature Request Request Technical Literature
Back to Oxford Biomedical Research Specific Products/Applications Oxford Biomedical Research Specific Products/Applications
Back to Oxford Biomedical Research Products and Applications Oxford Biomedical Research Products and Applications
Go to Xxidative Stress Feature Oxidative Stress Feature
tPA Products
Tissue-type Plasminogen Activator (tPA) Proteins, Antibodies and Assays
Go to Oxford Biomedical Research websitetPA is a fibrin specific activator of plasminogen that is produced in endothelial cells with a normal concentration of 5-10 ng/mL in plasma. The fibrin specificity is the result of the interaction of the kringle-2 domain of tPA with specific lysine residues on fibrin.

Nascent tPA is comprised of a single polypeptide chain. Its proteolytic cleavage at a centrally located arginine-isolucine bond by plasmin gives rise to a 2-chain form, composed of the N-terminal derived heavy chain and the C-terminal light chain that are linked by a disulfide bond. The majority of tPA in circulation is present as a complex with PAI-1. PAI-1 acts as an inhibitor to tPA by binding to tPA and thus stifling its ability to activate plasmin. tPA activity is an indicator of both myocardial infarction for patients with impaired fibrinolytic systems as well as a marker for type-II diabetes.

Recombinant tPA is produced  as an active single chain molecule with a molecular weight of approximately 70 kDa which can be cleaved by fibrin to the more active 2-chain form.
Click on the links below to generate a list of the tPA related products:
Go to list of tPA related products Tissue-type Plasminogen Activation (tPA)
uPA Products
Urokinase-type Plasminogen Activation (uPA) Proteins, Antibodies and Assays
Go to Oxford Biomedical Research websiteUrokinase-type Plasminogen Activator (uPA), also referred to as Urokinase, rapidly cleaves plasminogen, yielding plasmin. The key role of uPA in the firbrinolytic cascade is rapidly inhibited by the major modulator of fibrinolysis, PAI-1. uPA, when complexed with urokinase-type Plasminogen Activator Receptor (uPAR), functions to degrade the basement membrane and peritumoral matrix to facilitate the migration of tumor cells.

There are two forms of uPA, a 31 kDA low molecular weight (LMW) form and a 55 kDa high molecular weight (HMW) form. Autoproteolysis of HMW urokinase results in LMW urokinase and an 18.5 kDa amino terminal fragment (ATF). The ATF has been shown to inhibit proliferation and invasion of cancer cells by binding to the uPA receptor.

Click on the links below to generate a list of the uPA related products:
Go to list of uPA related products Urokinase-type Plasminogen Acitvation (uPA)
Go to top
Click on the links below to generate a list of other Vascular Biology related products:
Coagulation Proteins:
Go to list of Factors related products Factors V, VII, VIII, IX, X, XI, XII, XIII
Go to list of Fibrinogen related products Fibrinogen
Go to list of Protein C related products Protein C
Go to list of Thrombin related products Thrombin
 
Extracellular Matrix:
Go to list of Elastase related products Elastase
Go to list of Fibrinogen related products Fibrinogen
Go to list of Fibronectin related products Fibronectin
Go to list of Nexin related products Nexin
Go to list of Protease Inibitor related products Protease Inhibitors
Go to list of Trypsin related products Trypsin
Go to list of Vitronectin related products Vitronectin
 Lipoproteins:
Go to list of LDL-Receptor Associated Protein (RAP) products LDL-Receptor Associated Protein (LRP)
Go to list of RAP (Receptor Associated Protein) products Receptor Associated Protein (RAP)
Go to list of Renin/Prorenin related products Renin/Prorenin
Go to list of VLDL Receptor related products VLDL Receptor
 
Plasminogen Activation:
Go to list of Plasmin & Plaminogen related products Plasmin and Plasminogen
Go to list of PAI-1 Related Products Plasminogen Activation Inhibitor-1 (PAI-1)
Go to list of PAI-1 Assays PAI-1 - Assays
Go to list of PAI-1 Antibodies PAI-1 - Antibodies
Go to list of tPA related products Tissue-type Plasminogen Activation (tPA)
Go to list of uPA related products Urokinase-type Plasminogen Activation (uPA)
Go to top

 

 

Website by Websta Works