New Product - pOET-1 Transfer Plasmid
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NEW Transfer Plasmid pOET-1™ from Oxford Expression Technologies:
  Go to other Oxford Expression Technologies Products:
Go to flashBAC Product Information flashBAC Baculovirus Protein Expression
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  Literature
Go to pOET-1 Technical Literature Download pOET-1™ Technical Literature
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  NEW Transfer Plasmid
Transfer Plasmid pOET-1™
Go to Oxford Expression Technologies websiteOxford Expression Technologies has announced the release of pOET-1, the first member of a new family of transfer plasmids that are designed to form the perfect complement to their existing flashBAC™ range of vectors. The gene of interest undergoes homologous recombination into the virus genome (eg  flashBAC™) from the transfer plasmid. Hence cloning into the transfer vector forms the essential first step in a baculovirus expression experiment. This new transfer plasmid offers a number of features that make it an ideal starting point for expression work:
   
• Small size (4541bp) - makes the cloning steps easy
• Directional cloning into vector possible using unique restriction sites
• Supports the expression of truncated proteins
• Perfect companion product to the flashBAC™ system
• Compatible with other baculovirus systems that use homologous recombination
Compatibility with Other Baculovirus systems
pOET-1 is compatible with any baculovirus system that utilises homologous recombination in insect cells including the flashBAC™ products from Oxford Expression Technologies (OET):
 
• flashBAC (OET)
• BacVector 3000 (Novagen)
• flashBACGOLD (OET)
• BacPAK6 (Clontech)
• flashBACULTRA (OET)
• BaculoGold (Clontech)
• flashBAC10 (OET)
• BaculoBright (Clontech)
• BacMagic (Novagen)
• ProGreen (AbVector)
• BacMagic2 (Novagen)
• ProFold (AbVector)
• BacVector 1000 (Novagen)
• ProEasy (AbVector)
• BacVector 2000 (Novagen)
• Sapphire (Orbigen)
  How pOET-1™ Transfer Plasmid works:
pOET-1 is a baculovirus transfer plasmid designed for high level expression of foreign genes under the powerful AcMNPV polyhedrin (polh) promoter. pOET-1 has a Col E1 origin of replication and an ampicillin resistance gene for selection in E. coli. The plasmid is smaller than other available transfer plasmids (4541 bp) which greatly facilitate the cloning steps. The polh sequences have been replaced by a multiple cloning site containing 14 unique restriction sites for insertion of the foreign gene in the correct orientation. The PacI site at the end of the MCS provides translational stop codons in all three reading frames for expression of truncated proteins. The AcMNPV sequences flanking the gene in the transfer plasmid MCS allow recombination with the viral DNA to insert the expression cassette into the polh locus.
 
pOET-1™ Transfer Plasmid Technical Literature
 Description Size File1
pOET-1 Transfer Plasmid Information Sheet 160KB Download Information Sheet
pOET-1 Transfer Plasmid Base Sequence Data Sheet 6KB Download Base Sequence Data Sheet
pOET-1 Transfer Plasmid MSDS 34KB Download pOET-1 MSDS
Note 1: Click on the Adobe Acrobat  icon to download the file
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  Price for the NEW pOET-1™ Transfer Vector from Oxford Expression Technologies
Item Code1 Product Description Pack Size List Price2
OE-200101 pOET-1 Transfer Plasmid DNA, 10µg Each $470.00
Note 1: Click on the item code to go to Online Prices and Ordering
Note 2: Prices are exclusive of GST and local packing, handling and delivery charges
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