| Products |
Product Description
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| Lightning-Link® Antibody Labelling Products |
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| Lightning-Link® the World’s easiest to use antibody labelling kits. |
| Direct labelling (conjugation) of primary antibodies, offers significant benefits over indirect detection using secondary antibodies. For instance, direct antibody labelling: |
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Circumvents problems of cross over and non-specific binding of secondary reagents. |
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Reduces the number of incubations and tedious wash steps. |
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Delivers a unique opportunity to study multi-protein complexes using antibodies generated from either the same or different species. |
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| The Lightning-Link® process - simply add purified antibody to the lyophilised mixture |
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| What Makes Lightning-Link® Unique? |
| Easy to Use: |
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Hands on time of only 30 seconds |
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Conjugation reaction takes ca 3 hours at 20-25°C |
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No column separation steps are required to remove unbound labels |
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Molecules to be labelled only need to have a free amine group for efficient conjugation |
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| Easy on your protein: |
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Conjugation is carried at physiological pH |
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Works with phosphate, HEPES, MES, MOPS and other amine free buffers |
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| Reliable Results: |
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As the entire conjugation reaction is carried out in one tube, antibody recovery is 100% and labelling efficiency is very high |
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Reagents used in Lightning-Link are designed to deactivate and are completely benign after the labelling reaction and will not affect downstream experiments |
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Labelled antibody can be used directly in Western Blotting (WB), Enzyme Immunoassays (EIA's), Immunohistochemistry (IHC), FACS Analysis and other applications which require labelled antibodies or other biomolecules |
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| Convenient Pack Sizes: |
| All Lightning-Link® Labelling kits are available in a number of convenient packs. The majority of kits are available in two or more of the following pack sizes: |
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10µg or 20µg per reaction, 3 reactions per kit
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100µg, 1 reaction per kit |
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100µg or 200µg per reaction, 3 reactions per kit
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1mg or 2mg per reaction, 1 reaction per kit
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| Lightning-Link® Rapid Antibody Labelling Products |
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| Lightning-Link® Rapid the World’s fastest and easiest to use antibody labelling kits. |
| The Lightning-Lin®k Rapid Antibody Labelling kits offer all the same advantages as standard Lightning-Link® kits, but now the labelled antibody is ready to use in just 20 minutes. |
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The Lightning-Link® Rapid process - simply add purified antibody to the lyophilised mixture and in 20 minutes, the antibody is labelled and ready to use:
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| Lightning-Link® Rapid is currently available with the following labels and kit sizes: |
| Available Labels: |
Kit Sizes: |
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Atto488, 565, 594 and 633 |
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Cy3 and Cy5 |
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DyLight® 350, 405, 488, 550, 594, 633, 650, 680, 755 and 800 |
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FluoProbe647H |
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Fluorescein
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Rhodamine |
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10µg or 20µg per reaction, 3 reactions per kit |
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100µg or 200µg per reaction, 3 reactions per kit |
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1mg or 2mg per reaction, 1 reaction per kit |
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| imm-Link™ Immunogen Linking Kits |
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| imm-Link™ Immunogen Linking Kits simplify the conjugation of carrier proteins to haptens: |
BSA, Ovalbumin and Keyhole Limpet Hemocyanin (KLH) stimulate a large immune response and are typically used as carrier proteins to generate antibodies against small antigens (peptides or organic compounds). For success the antigen, normally referred to as a hapten, needs to be covalently linked to the carrier protein.
Specific imm-Link™ Immunogen Linking Kits are designed for haptens with either sulfhydryl, carboxyl or amines functional groups which are conjugated to a carrier protein simply by adding a solution of the hapten to a proprietary lyophilised mixture containing the carrier protein and all the required conjugation chemistry. The hands-on time to set up the conjugation reaction is typically 20-30 seconds.
Following successful conjugation the conjugate is dialysed using the supplied dialysis cartridge to remove unwanted by products. The hapten-carrier protein conjugate can then be easily recovered and is ready for use. |
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| All the imm-Link™ Immunogen Linking Kits are simply to use: |
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| What Makes imm-Link™ Immunogen Linking Kits Unique? |
| Easy to Use: |
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All components supplied in one kit. |
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Wide range of chemistries. |
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Hands on time to set up conjugation reaction is 20-30 seconds. |
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Supplied dialysis cartridge ensures conjugates are recovered in high yield. |
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| Available in 9 combinations of carrier proteins and conjugation chemistries: |
| Three Carrier Proteins: |
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BSA |
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Ovalbumin |
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Keyhole Limpet Hemocyanin (KLH) |
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| Three Conjugation Chemistries: |
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imm-Link™ Sulfhydryl Immunogen Kit covalently binds the carrier protein to cysteines or free sulfhydryl groups (R-SH) on the hapten |
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imm-Link™ Carboxyl Immunogen Kit covalently binds the carrier protein to Glutamic Acid, Aspartic Acid or to free carboxyl groups (R-COOH) on the hapten |
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imm-Link™ Amine Immunogen Kit covalently binds the carrier protein to Lysines or free amines (R-NH2) on the hapten |
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| Activated Labels |
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| Maleimide and Periodate Activated Labels |
Maleimide activated labels are designed to facilitate the conjugation of the desired label to either antibodies, proteins, peptides or ligands that contain a sulfhydryl (R-SH) group.
Periodate activated horseradish peroxidase (HRP) is designed to facilitate the conjugation of HRP to proteins, peptides and ligands that contain amine (-NH2) groups.
The reactive groups that have been added to the label allows conjugation reactions to be performed very efficiently at physiological pH for superior performance. |
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| Convenient and Easy to Use: |
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Eight maleimide activated labels and one periodate activated label are available |
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Supplied as freeze dried formulations, therefore shipping on dry ice is unnecessary |
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Can be used to label proteins, peptide and ligands |
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Labelling performed at physiological pH |
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| Available Maleimide Activated Labels: |
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Horseradish Peroxidase (HRP) |
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Alkaline Phosphatase (AP)
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R-Phycoerythrin (R-PE)
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Allophycocyanin-XL |
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Streptavidin |
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Ovalbumin |
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BSA |
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Keyhole Limpet Hemocyanin (KLH) |
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| Periodate Activated Label: |
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Horseradish Peroxidase (HRP) |
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| Antibody Purification |
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Antibody Purification Systems
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| The AbSelect range of antibody purification systems are cost effective and easy to use antibody purification systems which have been specifically designed to be compatible with a wide range of matrices. All the components of all the AbSelect™ kits are compatible with the Lightning-Link® Antibody Labelling kits, hence the purified antibodies can be immediately conjugated with the desired label. |
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| AbSelect™ Antibody Purification System |
| Used to purify commerciallyavailable antibodies which often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labelling reactions. The AbSelect™ Purification Kit quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. |
| Convenient and Easy to Use: |
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Suitable for volumes of 100µL to 0.5mL |
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Up to 500µg of antibody can be purified in each run |
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| AbSelect™ G Antibody Purification System |
| Used to purify commercially available antibodies which often contain substances (e.g. BSA, glycine, tris and azide) that interfere in labelling reactions with enzymes, biotin, streptavidin or fluorophores. The AbSelect™ G Purification System quickly removes these contaminants. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. |
| Convenient and Easy to Use: |
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Suitable for volumes of 100µL to 0.5mL |
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Up to 500µg of antibody can be purified in each run |
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| AbSelect™ G Serum Antibody Purification System |
| Antibodies generated from ascites fluid and serum are often supplied as crude formulations. Both ascites fluid and serum contain many substances that interfere with antibody labelling reactions. Successful labelling therefore requires antibodies to be purified prior to labelling. The AbSelect™ G Serum system is a fast and simple method to purify antibodies from these types of media. Protein G has a high affinity for the Fc regions of IgG molecules from a variety of species. |
| Convenient and Easy to Use: |
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Used to purify IgG from both ascites and serum |
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Upto 10mg of antibody can be purified in each run |
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| AbSelect™ Mouse Antibody Purification System |
| Use to purify commercially available antibodies often contain substances (e.g. BSA, glycine, tris and azide) that interfere in labeling reactions with enzymes, biotin, streptavidin or fluorophores. The AbSelect™ Mouse Antibody Purification System quickly removes these contaminants. It can also be used to purify mouse antibodies from crude samples such as ascites fluid. |
| Convenient and Easy to Use: |
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Suitable for volumes of 100µL to 0.5mL |
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20µ to 150µg of antibody can be purified in each run |
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| AbSelect™ Serum Antibody Purification System |
| Used to purify antibodies generated from ascites fluid and serum which are often supplied as crude formulations which contain many substances that interfere with antibody labelling reactions. |
| Convenient and Easy to Use: |
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Suitable for volumes of 4mL to 40mL of ascites fluid |
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Suitable volume for serum is species dependent |
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Upto 20mg of antibody can be purified in each run |
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| AbSelect™ Mouse TCS Purification System |
| Used to purify mouse IgG fractions from hybridoma supernatants. The method involves capture of the antibody on the AbSelect™ Mouse TCS resin which has a high affinity for mouse IgG and the removal of unwanted substances using a simple wash procedure. The purified product is then eluted and neutralized. The AbSelect™ Mouse TCS Purification System is not suitable for use with antibodies from other species. It should be noted that the binding strength of Bovine IgG to the AbSelect™ Mouse resin is negligible. |
| Convenient and Easy to Use: |
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Suitable for volumes of 10mL to 25mL of tissue culture supernatant. |
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Upto 1.5mg of antibody can be purified in each run |
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| AbSelect™ Tissue Culture Supernatant (TCS) Antibody Purification System |
| Usd to purify antibodies which are generated from hybridoma cell lines and supplied in tissue culture supernatant which contains many substances that interfere with antibody labelling reactions. |
| Convenient and Easy to Use: |
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Suitable for volumes of 10mL to 50mL of tissue culture supernatant. |
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Up to 5mg of antibody can be purified in each run |
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Products and Applications 
