New from Geneaid Biotech

New and Featured Products from Geneaid Biotech:

NEW and Featured Products

	NEW Reverse Protein Stain Kit
	High Speed Plasmid Purification
	Gel and PCR Fragment Extraction
	Prices of New and Featured Products

Literature

Download Geneaid Biotech Technical Literature

	Geneaid Biotech Specific Products/Applications
	Geneaid Biotech Products and Applications

Rapid Protein Staining of Electrophoresis Gels

NEW Reverse Protein Stain Kit

Reverse Protein Stain Kit uses imidazole and zinc salts for protein detection (as low as 1 ng) in electrophoresis gels. The method is based on selective precipitation of a white imidazole–zinc complex in the gel, except in those zones where proteins are located. These zones remain transparent. When the gel is placed above a dark background, the negative gel image can be converted to a positive image with black bands/spots and a white background. This stain is almost as sensitive as most silver stains and requires only 10 minutes to complete the staining proceedure. In addition, fixation of proteins to the gel is not needed, there is no interaction of the stain with the protein, and complete destaining of the matrix can be achieved. Imidazole-zinc reverse stain uses only two staining solutions, which can be easily diluted from stock solutions. Gels are typically stained immediately after electrophoresis. Finally, imidazole-zinc reverse stain is fully compatible to down-stream applications, such as mass spectrometry, Edman sequencing, electroelution, and membrane blotting techniques.

Advantages of Reverse Protein Stain Kit

The Reverse Protein Stain Kit from Geneaid Biotech offer the following advantages:

	•	Rapid Staining: <10 minutes compared to conventional staining methods which can require up to 24 hours
	•	High Sensitivity: can detect proteins down to 1ng
	•	Will not affect the proteins in the gel: integrity of the proteins are maintained
	•	Can be completely removed from gel: other staining methods can be used direclty following this procedure
	•	Room temperature storage: reagents stable for up to 2 years.

Geneaid Biotech Literature

Description	Size	File¹
Reverse Protein Stain Kit - Protocol	171KB
Note 1: Click on the Adobe Acrobat icon to download the file

Reduce the Cost of Plasmid Purification

High Speed Plasmid Purification

The High-Speed Plasmid Mini Kit was designed for rapid isolation of plasmid or cosmid DNA from 1-4 ml of cultured bacterial cells. Modified Alkaline Lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE. Typical yields are 20-30 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 30 minutes.

Geneaid Biotech Literature

Description	Size	File¹
High-Speed Plasmid Mini Kit - Protocol	254KB
96-Well Plasmid Kit - Protocol	244KB
Note 1: Click on the Adobe Acrobat icon to download the file

Equivalent Results When Compared to a Competitor's Product

In a recent comparison with a leading brand the Geneaid Biotech High Speed Plasmid Mini Kit produced equivalent results as shown below:

A sample of bacterial cell culture was processed using the Geneaid Biotech High Speed Plasmid Mini Kit and the equivalent competitor's product.

Test Plasmid: pBluescript (3kb)
Test Volume: bacterial culture (1.5 mL)
Elution Volume: 50μL
M: 1kb DNA ladder

Product Source	Test	260/ 280	260/ 230	Conc (µg/mL)
Competitor	Lane 1	1.88	2.25	8.3
Competitor	Lane 2	1.88	2.24	8.4
Geneaid	Lane 3	1.87	2.21	8.3
Geneaid	Lane 4	1.87	2.23	8.5

Conclusion: In terms of Yield, OD Values and Quality of DNA, the Geneaid Biotech High Speed Plasmid Mini Kit produce equivalent results to the competitor's kit.

Reduce the Cost DNA Fragment Extraction

Gel and PCR DNA Fragment Extraction

The Gel/PCR DNA Fragments Extraction Kit was designed to recover or concentrate DNA fragments (50 bp→10 Kb) from agarose gel, PCR, or other enzymatic reactions. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation.

Geneaid Biotech Literature

Description	Size	File¹
Gel/PCR DNA Fragment Extraction Kit - Protocol	266KB
96-Well Gel/PCR DNA Extraction Kit - Protocol	228KB
Note 1: Click on the Adobe Acrobat icon to download the file

Equivalent Results When Compared to a Competitor's Product

In a recent comparison with a leading brand the Geneaid Biotech Gel/PCR DNA Fragment Extraction Kit produced equivalent results as shown below:

Following PCR amplification of a DNA (3.7kb), the amplified DNA was extracted using both the Geneaid Biotech Gel/PCR DNA Fragment Extraction Kit and the equivalent competitor's kit.

Lane	Test	Yield (µg/mL)	260/280	260/230
1	Original	-	-	-
2	Geneaid	5.1	1.87	1.65
3	Competitor	5.0	1.87	0.52

DNA fragments <5kb in size were cut to test the gel extraction efficiency of both the Geneaid Biotech Gel/PCR DNA Fragment Extraction Kit and the equivalent competitor's kit.

G=Geneaid; M=MW Ladder; C=Competitor

Conclusion: The argarose gel at left and above show the clearly the high quality of both the Geneaid Biotech Gel/PCR DNA Fragment Extraction Kit and the equivalent competitor's kit.

Opposite right: The data from ligation and sequencing experiements using both the Genaid Biotech and the competitor's kits further illustration the quality of the Genaid Biotech kits.

Click on the image to view full size.

Ligation

Sequencing