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Geneaid Biotech - Specific Application Areas
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Specific Application and Product Information on the Geneaid Biotech Range of Products
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Information on Specific Application Areas for Geneaid Biotech products can be found below. For full information on any Geneaid Biotech products, please visit the Geneaid Biotech website.
Specific Product/Application Areas:
Go to Genomic DNA Purification Kits Genomic DNA Purification Kits
Go to Plasmid/Cosmid Purification Kits Plasmid Purification Kits
Go to Gel/PCR Fragment Extraction Kits Gel/PCR Fragment Extraction Kits
Go to RNA Purification Reagents and Kits RNA Purification Reagents and Kits
Go to Viral Nucleic Acid Purification Kits Viral DNA/RNA Purification Kits
Go to Nucleic Acid Isolation Kit and Reagents Nucleic Acid Isolation Kits and Reagents
Go to Magnetic Bead DNA/RNA Purification Kits Magnetic Bead DNA/RNA Purification Kits
Go to NEW and Featured Products NEW and Featured Products
Go to NEW Reverse Protein Stain Kit NEW Reverse Protein Stain Kit
Go to High Speed Plasmid Purification High Speed Plasmid Purification
Go to Gel and PCR DNA Fragment Extraction Gel and PCR DNA Fragment Extraction
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Specific Product/Application Areas Product and Applications
Genomic DNA Purification Kits
 
Go to Geneaid websiteAfter pretreatment, which depends on the sample type, chaotropic salt (and Proteinase K where required by the protocol) are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fibre matrix. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE.
The Genomic DNA Purification Kits are available in the following formats:
     
  • The DNA Pure Kit was designed to purify or concentrate DNA products which have been previously isolated using Geneaid’s Reagents (DAabsolute, Plant DNabsolute and Reagent Genomic DNA Kit) or any other isolation method.
Sample Size: up to 100µL of DNA products
  • The Genomic DNA Purification Mini/Midi/Maxi Kits (Blood/Cultured Cell)2 provides an efficient method for total DNA purification, including genomic DNA purification, mitochondrial DNA purification and virus DNA purification from whole blood, frozen blood, buffy coat, cultured animal/bacterial cells and fungi.
Sample Size:
Mini Kit - up to 300µL of whole blood, up to 200µL of frozen blood, up to 200µL of buffy coat
Midi Kit - up to 2mL of frozen blood, cultured cells (up to 5 x 107 cells)
Maxi Kit - up to 10mL of frozen blood, cultured cells (up to 1 x 108 cells)
  • The Genomic DNA Purification Mini Kit (Tissue)3 was designed specifically for total DNA purification, including genomic DNA purification, mitochondrial DNA purification and virus DNA purification from a variety of animal tissue, paraffin-embedded tissue, buccal swab and amniotic fluid.
Sample Size: up to 30mg of tissue, up to 25mg of paraffin-embedded tissue, buccal swab, up to 15mL of amniotic fluid
  • The gSYNC Mini Kit1 synchronizes the Genomic DNA Mini Kit (Blood/Cultured Cell) and Genomic DNA Mini Kit (Tissue) into one convenient product. The operation time is dramatically reduced, making the gSYNC Mini Kit a very efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from whole blood (fresh/frozen blood), cultured animal/bacterial cells, fungus and tissue.
Sample Size: 50-200µL whole blood, up to 200µL fresh blood, cultured cells (up to 1 x 107 cells), bacterial cells (up to 1 x 109 cells), fungus cells (up to 5 x 107 cells), up to 25mg tissue
  • The 96-Well Genomic DNA Purification Kit (Plant)4 and the Genomic DNA Purification Mini/Maxi Kit (Plant)4 provides an efficient method for total DNA purification, including genomic DNA purification, mitochondrial DNA purification and chloroplast DNA purification from plant tissue.
Samples Size:
96-Well Kit - up to 100mg of fresh, up to 25mg of dry plant tissue
Mini Kit - up to 100mg (fresh plant tissue), up to 25mg (dry plant tissue)
Maxi Kit - up to 1g (fresh plant tissue), up to 250mg (dry plant tissue)
  • The 96-Well Genomic DNA Purification Kit5 was designed for high-throughput total DNA purification, including genomic DNA purification, mitochondrial DNA purification and virus DNA Purification from whole blood, animal tissue, cultured cells and fungi.
Samples Size: up to 200µL of blood, up to 20mg of tissue, cultured cells (up to 1 x 107 cells), bacterial cells (up to 1 x 109 cells), fungal cells (up to 5 x 107 cells)
     
    The purified nucleic acids are suitable for:
    Note 1: PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation
    Note 2: PCR or other enzymatic reactions
    Note 3: PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
    Note 4: PCR, Real-time PCR, Southern Blotting and RFLP
    Note 5: PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
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Plasmid Purification Kits
 
Go to Geneaid websiteThe Plasmid DNA Purifcation kits use a modified Alkaline Lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fibre matrix. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE. In the case of the Plasmid Midi and Maxi kits and corresponding Endotoxin Free kits, the Plasmid DNA is bound to an anion exchange resin instead of a glass fibre matrix. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the anion exchange resin in the column and the contaminants are removed with the PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting.
Plasmid DNA Purification Kits are available in the following formats:
     
  • The High-Speed Plasmid Mini Kit1 is designed for the rapid isolation of plasmid DNA from bacterial cultures.
Sample Size: 1-4mL of bacterial cultures
  • The High-Speed Plasmid Advance Kit1 was designed for rapid purification of plasmid or cosmid DNA from 50-100 mL of cultured bacterial cells.
Sample Size: High copy number plasmid 20-50mL of bacterial culture, Low copy number plasmid 50-150mL of bacterial culture.
  • The Plasmid Midi/Maxi Kits use pre-packed anion-exchange resin columns for plasmid DNA purification from bacterial cultures.
Samples Size: 50-250mL of cultured bacterial cells
  • The Plasmid Midi/Maxi Kits (Endotoxin Free)2 uses pre-packed anion-exchange resin columns to purify plasmid DNA from cultured bacterial cells.
Sample Size:
Midi Kit - 50-100mL of cultured bacterial cells
Maxi Kit - 150-250mL of cultured bacterial cells
  • The 96-Well Plasmid Kit3 was designed for high-throughput plasmid DNA purification from cultured bacterial cells.
Sample Size: 1-2mL of cultured bacterial cells
     
    Note 1: Restriction Enzyme digestion, Library Screening, Ligation, PCR, Transformation/Sequencing Reactions
    Note 2: Transfection, Sequencing, Ligation, PCR, In-vitro Transcription, Microinjection, Restriction Enzyme Digestion, Gene Gun
    Note 3: Fluorescent/Radioactive Sequencing, Restriction Enzyme Digestion, Library Screening, Ligation, Transformation and PCR
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Gel/PCR Fragment Extraction Kits
 
Go to Geneaid websiteChaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fibre matrix. Contaminants are removed with a Wash Buffer containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE.
The Gel/PCR Fragment Extraction kits are available in the following two formats:
     
  • The Gel/PCR DNA Fragments Extraction Kit1 and Gel/PCR DNA Fragments Extraction Maxi Kit1 are designed to recover or concentrate DNA fragments (50bp–10kb) from agarose gel, PCR, or other enzymatic reactions.
Samples Size:
Standard Kit - up to 300mg agarose gel slice, up to 100μL (PCR products)
Maxi Kit - up to 6g of agarose gel, up to 2mL of PCR products
  • The 96-Well Gel/PCR DNA Extraction Kit1 provides a high-throughput, rapid and economical method to purify DNA fragments.
Samples Size: up to 50mg of agarose gel slice, up to 50μL of PCR products
  • The Small DNA Fragments Extraction Kit1 was designed to recover or concentrate DNA fragments (50-200bp) from agarose gel, PCR, or other enzymatic reactions.
Sample Size: up to 300 mg (agarose gel slice), up to 100 μL (PCR products)
  • The Large DNA Fragments Extraction Kit1 was designed to recover or concentrate DNA fragments (>8kb) from agarose gel.
Sample Size: up to 300mg agarose gel slice
     
    Note 1: The extracted nucleic acids are suitable for PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA labelling and Ligation
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RNA Purification Kits
 
Go to Geneaid websiteChaotropic salt, (some kits also use a detergent) is used to lyse cells and inactivate RNase allowing RNA to be easily bound by the glass fibre matrix. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified RNA is eluted by RNase-free water.
The RNA Purification Kits are available in the following formats:
     
  • The RNA Pure Kit1 was designed for RNA purification or RNA concentration from products which have been previously isolated using Geneaid’s Reagents (RNabsolute andPlant RNabsolute) or any other isolation method.
Sample Size: up to 100µL of RNA products
  • The Total RNA Purification Mini/Maxi Kit (Blood/Cultured Cell)2 was designed specifically for total RNA purification from fresh whole human blood and cultured cells.
Samples Size:
Mini Kit - up to 300µL of whole blood, cultured cells (up to 5 x 106 cells) bacterial cells (up to 1 x 109 cells) fungal cells (up to 5 x 107 cells)
Maxi Kit - up to 5mL of whole blood, cultured cells (up to 1 x 108 cells), bacterial cells (up to 1 x 1010 cells)
  • The Total RNA Purification Mini/Maxi Kits (Tissue)3 was designed specifically for  total RNA purification from a variety of animal and paraffin-embedded tissue samples.
Sample Size:
Mini Kit - up to 25mg of tissue, up to 25mg of paraffin-embedded tissue
Maxi Kit - 100-200mg of tissue, cultured cells (up to 1 x 108 cells)
  • The Total RNA Purification Mini/Maxi Kits (Plant)4 provides an efficient method for total RNA purification from plant tissue and cells.
Samples Sizes:
Mini Kit - up to 100mg (fresh plant tissue) or up to 25 mg (dry plant tissue)
Maxi Kit - up to 500mg (fresh plant tissue)
  • The miRNA Isolation Kit was specifically designed for purification of small RNA with minimal contamination from large RNA molecules or genomic DNA.
Sample Size: 100mg (tissue), cultured cells (up to 1 x 106 cells)
  • The 96-Well Total RNA Purification Kit5 was designed specifically for high-throughput total RNA purification from cultured animal cells. However, RNA can also be efficiently purified from blood, bacteria, fungi and tissue samples.
Sample Size: cultured animal cells (up to 5 × 106 cells), up to 300μL of whole blood, cultured bacterial cells (up to 1 x 109 cells) fungal cells (up to 5 x 107 cells) up to 25mg of tissue, up to 25mg of paraffin-embedded tissue
     
    The purified nucleic acids are suitable for:
    Note 1: PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation
    Note 2: RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis
    Note 3: RT-PCR, Northern Blotting, Primer Extension, mRNA Selection, cDNA Synthesis, RNase Protection Assay
    Note 4: RT-PCR, Northern Blotting, Real-time PCR
    Note 5: RT-PCR, Real-time PCR, Northern Blotting, mRNA Selection, Microarrays, cDNA Synthesis
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Viral DNA/RNA Purfication Kits
 
Go to Geneaid websiteDNA/RNA viruses are lysed quickly and efficiently when exposed to the lysis buffer which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates optimum conditions for Nucleic Acid binding to the glass fibre matrix. Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash step. The nucleic acid can be eluted in RNase-free water and is then ready for use in subsequent reactions
The Viral RNA/DNA Kits are available in the following formats:
     
  • The Virus DNA/RNA Extraction Kit II1 was designed specifically for virus DNA/RNA purification from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures (H1N1, HIV/AIDS, HPV, HBV, etc.).
Sample Size: 200µL samples of plasma, serum, body fluids, supernatant of viral infected cell cultures
  • The Virus DNA/RNA Extraction Kit III1 was designed specifically for simultaneous virus DNA/RNA purification from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures (H1N1, HIV/AIDS, HPV, HBV, etc.).
Samples Size: 1mL of serum or plasma
  • The 96-Well Virus DNA/RNA Extraction Kit1 was designed specifically for high-throughput virus DNA/RNA purification of from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures (H1N1, HIV/AIDS, HPV, HBV, etc.).
Sample Size: 200µL samples of plasma, serum, body fluids, supernatant of viral infected cell cultures
     
    Note 1: The extracted viral DNA/RNA are suitable for RT-PCR/PCR, Real-time PCR/Real-time RT-PCR, Automated Fluorescent DNA Sequencing
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Nucleic Acid Isolation Kits & Reagents
 
Go to Geneaid websiteThese kits and reagents provide a fast and efficient method of isolating total RNA and DNA from a variety of sample types and sample sizes.
The Nucleic Acid Isolation Kit & Reagents are available in the following formats:
     
  • The Reagent Genomic DNA Isolation Kit1 was designed specifically for genomic DNA isolation from large volumes of blood. However, this highly versatile reagent system is able to extract genomic DNA from various other samples, such as cultured cells, tissue, bacteria and fungi.
Sample Size: ≥50µL of blood and buffy coat, cultured cells (up to 1 x 107 cells), bacteria (up to 1 x 109 cells), fungi (up to 5 x 107 cells), up to 20mg of tissue
  • DNabsolute3 (formerly Tri-Total Nucleic Acid Isolation Reagent Kit) provides a quick and easy 3 step method for total DNA/RNA isolation from tissue, cultured animal and bacterial cells, blood and serum.
Sample Size: 50mg (fresh tissue), 1.5-3mL (bacterial cells), cultured cells (up to 5 x 106 cells), 300µL (blood or serum)
  • Plant DNabsolute2 (formerly Tri-Plant Genomic DNA Reagent Kit) provides a quick and easy 3 step method for total DNA isolation (including genomic DNA isolation, mitochondrial DNA isolation and chloroplast DNA isolation) from plant tissue and plant cells.
Sample Size: 100mg (fresh plant tissue)
  • RNabsolute4 (Formely Tri-RNA Isolation Reagent) provides an efficient 3 step method for total RNA isolation from tissue, cultured animal and bacterial cells, blood and serum.
Sample Size: 50mg (fresh tissue), 1.5-3mL (bacterial cells), cultured cells (up to 5 x 106 cells), 300µL blood or serum
  • Plant RNabsolute4 (formerly Tri-Plant RNA Isolation Reagent) provides a quick and easy 3 step method for total RNA isolation  from plant samples.
Sample Size: 100mg (plant tissue)
  • The 96-Well DNabsolute3 (formerly 96-Well Tri-Nucleic Acid Isolation Reagent Kit) provides an efficient method for total DNA/RNA isolation from tissue, cultured animal and bacterial cells, blood and serum.
Sample Size: 50mg (fresh tissue), 1.5-3mL (bacterial cells), cultured cells (up to 5 x 106 cells), 300µL (blood or serum)
  • The 96-Well Plant DNabsolute2 (formerly 96-Well Tri-Plant Genomic DNA Isolation Reagent Kit) provides an efficient method for total DNA isolation (genomic DNA isolation, mitochondrial DNA isolation and chloroplast DNA isolation) from plant tissue and cells.
Sample Size: up to 50mg (fresh plant tissue)
     
    The isolated nucleic acids are suitable for:
    Note 1: PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR
    Note 2: PCR, Real-time PCR, Southern Blotting, Mapping and RFLP
    Note 3: PCR, RT-PCR, Southern Blotting, Northern Blotting, Mapping and RFLP
    Note 4: RT-PCR, Northern Blotting, cDNA Synthesis and Mapping

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Magnetic Bead DNA/RNA Purification Kits
 
Go to Geneaid websiteThe MagBead Kits are designed specifically for isolating Nucleic Acids from a variety of sampe types. The unique buffer system and chaotropic salts are used to lyse cells and degrade protein, allowing for the Nucleic Acid to be easily bound by the surface of the magnetic particles. Unwanted Nucleic Acids and other non-specific binding particles are removed with a Wash Buffer, and the desired Nucleic Acid is then released into the release buffer. Manual sample processing can be completed within 45 minutes (using most magnetic separators) or the kits can be easily adapted to satisfy most automated Nucleic Acid purification systems
MagBead DNA/RNA Purification Kits are available in the following formats:
     
  • MagBead Plant Genomic DNA Kit1,2
  • MagBead Buffy Coat Genomic DNA Kit1
  • MagBead Whole Blood/Cultured Cell Genomic DNA Kit1
  • MagBead Bacteria Genomic DNA Kit1
  • MagBead Tissue Genomic DNA Kit1
  • MagBead Viral DNA/RNA Kit1,2,3
     
    Note 1: The purified nucleic acids are suitable for Restriction Enzyme Digests, Southern Blots, PCR and qPCR assays.
    Note 2: The purified nucleic acids are suitable for qRT-PCR assays
    Note 3: For isolation from serum or plasma samples
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