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Geneaid Biotech - Specific Application Areas
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Specific Application and Product Information on the Geneaid Biotech Range of Products
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Information on Specific Application Areas for Geneaid Biotech products can be found below. For full information on any Geneaid Biotech products, please visit the Geneaid Biotech website.
Specific Product/Application Areas:
Go to Magnetic Bead DNA/RNA Purification Kits Magnetic Bead DNA/RNA Purification Kits
Go to RNA Purification Reagents and Kits RNA Purification Reagents and Kits
Go to Plasmid/Cosmid Purification Kits Plasmid/Cosmid Purification Kits
Go to Viral Nucleic Acid Purification Kits Viral Nucleic Acid Purification Kits
Go to Gel/PCR Fragment Extraction Kits Gel/PCR Fragment Extraction Kits
Go to Nucleic Acid Isolation Kit and Reagents Nucleic Acid Isolation Kits and Reagents
Go to Genomic DNA Purification Kits Genomic DNA Purification Kits
 
Go to Featured ProductsFeatured Products
Go to New Products High Speed Plasmid Purification
Go to New Products Gel and PCR DNA Fragment Extraction
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Specific Application Areas Product and Applications
Magnetic Bead DNA/RNA Purification Kits
 
Go to Geneaid websiteThe MagBead Kits are designed specifically for isolating Nucleic Acids from a variety of sampe types. The unique buffer system and chaotropic salts are used to lyse cells and degrade protein, allowing for the Nucleic Acid to be easily bound by the surface of the magnetic particles. Unwanted Nucleic Acids and other non-specific binding particles are removed with a Wash Buffer, and the desired Nucleic Acid is then released into the release buffer. Manual sample processing can be completed within 45 minutes (using most magnetic separators) or the kits can be easily adapted to satisfy most automated Nucleic Acid purification systems
MagBead DNA/RNA Purification Kits are available in the following formats:
     
  • MagBead Plant Genomic DNA Kit1
  • MagBead Buffy Coat Genomic DNA Kit1
  • MagBead Whole Blood/Cultured Cell genomic DNA Kit1
  • MagBead Bacteria Genomic DNA Kit1
  • MagBead Tissue Genomic DNA Kit1
  • MagBead Viral DNA/RNA Kit2
     
    The purified nucleic acids are suitable for:
    Note 1: The genomic DNA can be used directly in Restriction Enzyme Digests, Southern Blots, PCR and qPCR assays.
    Note 2: The viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.










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Plasmid/Cosmid Purification Kits
 
Go to Geneaid websiteThe Plasmid DNA Purifcation kits use a modified Alkaline Lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fibre matrix used. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer or TE. In the case of the Plasmid Midi and Maxi and corresponding Endotoxin Free kits, the Plasmid DNA is bound to an anion exchange resin instead of a glass fibre matrix. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the anion exchange resin in the column and the contaminants are removed with the PW Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting.
Plasmid and Cosmid DNA Purification Kits are available in the following formats:
     
  • The High-Speed Plasmid Mini Kit is designed for the rapid isolation of plasmid or cosmid DNA from 1-4mL of bacterial cultures1
  • The HigH Speed Plasmid Advance Kit is designed for the rapid isolation of plasmid or cosmid DNA from 50-100mL of bacterial culture1
  • Plasmid Midi2 and Maxi3 Kits for ultra-pure and large-quantity plasmid preparation4
  • Plasmid Midi and Maxi Endotoxin Free Kits for ultra-pure and large-quantity plasmid preparation4
  • 96-Well Plasmid Kit for high throughput isolation of plasmid or cosmid DNA from 1-2mL of bacterial cultures1
     
    The purified nucleic acids are suitable for:
    Note 1: The purified plasmid DNA is ready to use for restriction enzyme digestion, ligation, PCR, and sequencing reactions.
    Note 2: High copy number plasmid 20-50mL of bacterial culture, Low copy number plasmid 50-150mL of bacterial culture.
    Note 3: High copy number plasmid 50-150mL of bacterial culture, Low copy number plasmid 150-300mL of bacterial culture.
    Note 4: The obtained plasmid DNA is suitable for transfection, sequencing reactions, PCR, and in-vitro transcription.
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Gel/PCR Fragment Extraction Kits
 
Go to Geneaid websiteChaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fibre matrix. Contaminants are removed with a Wash Buffer containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer or TE.
The Gel/PCR Fragment Extraction kits are available in the following two formats:
     
  • The Gel/PCR DNA Fragments Extraction Kit1 is designed to recover or concentrate DNA fragments (50 bp–10 kb) from agarose gel, PCR, or other enzymatic reactions. Typically, recoveries are 80-90% for Gel Extraction and 90-95% for PCR Clean up. The entire procedure can be completed in 20 minutes and the eluted DNA is ready to use.
  • The 96-Well Gel/PCR DNA Extraction Kit provides a high-throughput, rapid and economical method to purify DNA fragments. Typically, recoveries are 80-90% for Gel Extraction and 90-95% for PCR Clean up. The entire procedure can be completed in 30-40 minutes and the eluted DNA is ready to use.
     
    The extracted nucleic acids are suitable for:
    Note 1: PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA labeling and Ligation
    Note 2: Restriction Enzyme Digestion, Ligation, PCR and Sequencing Reactions
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Genomic DNA Purification Kits
 
Go to Geneaid websiteAfter pretreatment, which depends on the sample type, chaotropic salt (and Proteinase K where required by the protocol) are used to lyse cells and degrade protein, allowing DNA to be easily bound by the glass fibre matrix. Once any contaminants have been removed, using a Wash Buffer (containing ethanol), the purified DNA is eluted by a low salt Elution Buffer or TE.
The Genomic DNA Purification Kits are available in the following formats:
     
  • Genomic DNA Mini/Midi/Maxi Kits (Blood/Cultured Cell)1 provides an efficient method for purifying total DNA (including genomic, mitochondrial and viral DNA) from a range of samples. Sample Size/Type: up to 300μL of whole blood, up to 200μL of frozen blood, up to 200μL of buffy coat, cultured animal cells (up to 1x107), cultured bacterial cells (up to 1x109) and fungus cells (up to 5x107)
  • Genomic DNA Mini Kit (Tissue)2 was designed specifically for purifying total DNA (including genomic, mitochondrial and viral DNA) from a variety of animal tissue, paraffin-embedded tissue, buccal swab and amniotic fluid. Sample size: up to 30 mg of tissue, up to 25 mg of paraffin-embedded tissue, buccal swab and up to 15 ml of amniotic fluid
  • Genomic DNA Mini Kit (Plant)3 provides a quick and easy method for purifying total DNA (including genomic, mitochondrial and chloroplast DNA) from plant tissue. Sample Size/Type: up to 100mg (Mini)/1g (Maxi) of fresh plant tissue and up to 25mg (Mini)/250mg (Maxi) of dry plant tissue
  • 96-Well Genomic DNA Kit4 was designed for high-throughput purification of total DNA (including genomic, mitochondrial and viral DNA). Sample Size/Type: up to 200μL of blood, up to 20mg of tissue, cultured cells (up to 1x107), bacterial cells (up to 1x109) and fungus cells (up to 5x107)
     
    The purified nucleic acids are suitable for:
    Note 1: PCR or other enzymatic reactions
    Note 2: PCR, AFLP/PADP, RFLP, Southern Blotting, Real-time PCR
    Note 3: PCR, Real-time PCR, Southern Blotting and RFLP
    Note 4: PCR, AFLP, RFLP, Southern Blotting, Real-time PCR
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RNA Purification Kits
 
Go to Geneaid websiteChaotropic salt, (some kits also use a detergent) is used to lyse cells and inactivate RNase allowing RNA to be easily bound by the glass fibre matrix. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified RNA is eluted by RNase-free water.
The RNA Purification Kits are available in the following formats:
     
  • The total RNA Mini/Maxi Kit (Blood/Cultured Cell)1 is specially designed for purification of total RNA from cultured cells and fresh whole human blood. Sample size: Mini - 5×106 animal cultured cells, 1×109 bacterial cells, or 300μL blood: Maxi - up to 5mL whole human blood up to 1x108 cultured mammalian cells up to 1x1010 bacterial cells
  • The total RNA Mini/Maxi Kit (Tissue)1 is specially designed for purification of total RNA from a variety of animal tissues and cells. Sample size: Mini - up to 25mg tissue, 5×106 animal cultured cells, 1×109 bacterial cells or 300μL blood; Maxi - 100-200mg animal tissue, 107-108 animal cultured cells, 5ml blood, 109-1010 bacteria cells
  • The Total RNA Mini/Maxi Kit (Plant)2 provides a fast and simple method to isolate total RNA from plant tissue and cells. Sample size: Mini - 500mg fresh plant tissue; Maxi - 500mg fresh plant tissue
  • The miRNA Isolation Kit is designed for purification of micro RNAs (miRNAs)3 and other small cellular RNAs from tissue samples or cultured cells. The use of various ethanol concentrations during the binding selective step control the size of the RNA bound to the glass fibre. Sample size: 100mg tissue or 1x106 cultured cells
  • The 96-Well Total RNA Kit4 is specially designed for high-throughput purification of total RNA from cultured cells including animal and bacterial cultures. Sample size: 5×106 animal cultured cells 1×109 bacterial cells
     
    The purified nucliec acids are suitable for:
    Note 1: RT-PCR, Real-time RT-PCR, Northern Blotting, Primer Extension RNase Protection Assays, mRNA Selection, cDNA Synthesis
    Note 2: RT-PCR, Real-time PCR and Northern Blotting
    Note 3: RNAs is required for accurate analysis of miRNA expression by qPCR or microarray analysis
    Note 4: RT-PCR, Real-time PCR, Northern Blotting mRNA Selection, Microarrays, cDNA Synthesis
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Viral Nucleic Acid Purfication Kits
 
Go to Geneaid websiteDNA/RNA viruses are lysed quickly and efficiently when exposed to the lysis buffer which is a highly concentrated solution of chaotropic salt. When combined with ethanol, the AD Buffer creates optimum conditions for Nucleic Acid binding to the glass fibre matrix. Contaminants such as salts, metabolites and soluble macromolecular cellular components are removed in the Wash step. The nucleic acid can be eluted in RNase-free water and is then ready for use in subsequent reactions
The Viral Nucleic Acid Kits are available in the following formats:
     
  • The viral Nucleic Acid Extraction Kit1 II is specially designed for high-throughput purification of viral RNA or DNA from cell-free samples as serum, plasma, body, fluids, and the supernatant of viral infected cell cultures. Sample size: 200µL
  • The Viral Nucleic Acid Extraction Kit2 III is designed for the simultaneous purification of viral DNA and RNA from 1 ml serum samples. Sample size: 1mL
  • The 96-Well Viral DNA/RNA Extraction Kit3 is specially designed for high-throughput purification of viral RNA or DNA from cell-free samples as serum, plasma, body fluids and the supernatant of viral infected cell cultures. Samples size 200µL
     
    The purified viral nucleic acids are suitable for:
    Note 1: RT-PCR/PCR, Real-time PCR/Real-time RT-PCR, Automated Fluorescent DNA Sequencing
    Note 2: RT-PCR/PCR, Real-time PCR/RT-PCR, Automated Fluorescent DNA Sequencing
    Note 3: Real-time, PCR/RT-PCR, Automated Fluorescent DNA Sequencing, PCR, and other enzymatic reactions
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Nucleic Acid Isolation Kits & Reagents
 
Go to Geneaid websiteThese kits and reagents provide and fast and efficient method of isolating total RNA and DNA from a variety of sample types and sample sizes.
The Nucleic Acid Isolation Kit & Reagents are available in the following formats:
     
  • Tri-Total RNA Isolation Reagent1 provides an efficient 3 step method to isolate total RNA from tissue, cultured animal and bacterial cells, blood and serum. The unique RAR Buffer system ensures total RNA with high yield and good quality from samples of unlimited size by scaling up the buffer volume.
  • The Tri-Plant Genomic DNA Reagent Kit2 provides a quick and easy 3 step method to isolate total DNA (including genome, mitochondrial and chloroplast DNA) from plant tissue and cells. Samples are initially disrupted by grinding in liquid nitrogen, followed by lysate treatment with RNase A. Sample size: up to 100mg of fresh plant tissue.
  • Tri-Plant RNA Isolation Reagent2 provides a quick and easy 3 step method to isolate total RNA from plant samples. The unique RPR Buffer system ensures total RNA with high yield and good quality from most common plant samples and also samples high in polysaccharides. If a larger sample is required, the buffer volume can be scaled proportionately.
  • Tri-RNA Isolation Reagent3 provides an efficient 3 step method to isolate total RNA from tissue, cultured animal and bacterial cells, blood and serum. The unique RAR Buffer system ensures total RNA with high yield and good quality from samples of unlimited size. If a larger sample is required, the buffer volume can be scaled proportionately.
  • The Reagent Genomic DNA Kit was designed specifically for DNA isolation from large volumes of blood. However, this highly versatile reagent is able to extract genomic DNA from various other samples, such as cultured cells, tissue, bacteria and fungus in samples <50µL
  • The 96-Well Plant Genomic DNA Reagent Kit2 provides a fast and simple method to isolate total DNA (genomic DNA, mitochondrial and chloroplast) from plant tissue and cells. Samples are broken down by grinding in liquid nitrogen and GR Lysis Buffer incubation. The lysate is treated with RNase A to degrade RNA
     
    The isolated nucleic acids are suitable for:
    Note 1: PCR, RT-PCR, Southern Blotting, Northern Blotting, Mapping and RFLP
    Note 2: PCR, Real-time PCR, Southern Blotting, Mapping and RFLP
    Note 3: RT-PCR, Northern Blotting, cDNA, Synthesis and Mapping
    Note 4: PCR, AFLP, RFLP/PADP, Southern Blotting, Real-time PCR

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