ChromoTek - Products and Applications
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ChromoTek range of innovative Nanobodies for Immunoprecipitation
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Nano-Traps are ideal for fast, reliable and efficient one-step immunoprecipitations. They pull down the proteins of interest (POI) and their interacting factors from cell extracts or organelles. Nano-Traps consist of a VHH, also termed a "nanobody", coupled to an immobilizing matrix (agarose beads, magnetic agarose beads, magnetic particles or multiwell plates).
 
Nano-Traps are available against:
Peptide/Protein Tags: Specific Proteins:
•  GFP-Trap •  Dmnt1-Trap
•  RFP-Trap •  Mdm4/ HdmX-Trap
•  Myc-Trap •  MK2-Trap
•  GST-Trap •  p53 N-term-Trap
•  MBP-Trap •  p53 C-term-Trap
    •  PARP1-Trap

GFP-Trap® and RFP-Trap® are well established high quality tools for the fastreliable and efficient one-step isolation of fluorescent fusion proteins and their interacting factors. (eg pulldown, immunoprecipitation, Co-IP, Western blot, mass spectrometry or ChIP).

The GFP-Trap® and RFP-Trap® feature:
  A robust and versatile tool for biochemical analyses of GFP fusion proteins or RFP fusion proteins
  Short incubation times (5-30 min)
  Quantitative isolation of fusion proteins and transiently bound factors from various cell extracts and organelles (from all organisms)
  No non-specific binding or contamination by heavy and light chains of conventional antibodies
  Functionality for Chromatin Immunoprecipitation (ChIP)
HOW TO FIND CHROMOTEK PRODUCTS:
By clicking on the next to "Price List" under the "Product/Applications Areas and Prices" links below, you will be taken to "Online Ordering" and a list of all ChromoTek products will be generated within the selected "Product/Application Area". From there the search can be further refined by selecting a "Product Group" and clicking on "Guided Search".

Alternatively, go directly to Online Ordering for a list of all ChromoTek products which can then be similarly searched by "Application Area" and then by "Product Group".

The SITE SEARCH function, at left, can also be used to find products. Enter the  product code or a full or a partial description of a product and click on "Seach". A list of ALL products within the Online Ordering database containing the item code or word(s) and/or keyword entered will be generated.
Product/Application Areas and Prices:
Go to GFP-Trap Products GFP-Trap Products
Go to GFP-Trap Products RFP-Trap Products
Go to GST-Trap Products GST-Trap Products
Go to MBP-Trap Products MBP-Trap Products
Go to Myc-Trap Products Myc-Trap Products
Go to Dnmt1-Trap Products Dnmt1-Trap Products
Go to MK2-Trap Products MK2-Trap Products
Go to p53-Trap Products p53-Trap Products
Go to p53-Trap Products PARP1-Trap Products
Go to Mdm4/ HdmX-Trap Products Mdm4/ HdmX-Trap Products
Go to Chromobody Products Chromobody Products
Go to Nano Boosters Nano Boosters
Go to Monoclonal Antibodies Monoclonal Antibodies
Go to Binding Controls Binding Controls
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Pricing and Availability: A$ Prices are on this website, alternatively, please contact us for prices and availability on any of the ChromoTek's range of products.
Go to ChromoTek Products/Application Areas Product/Application Areas and Prices:
Go to Specific Products/Application Areas Specific Products/Application Areas
Go to Technical Overview - NanoTrap Technology Technical Overview - NanoTrap Technology
Go to GFP-Trap Products GFP-Trap Products
Go to RFP-Trap Products RFP-Trap Products
Go to GFP-multiTrap Products GFP-multiTrap
Go to GFP-Booster Products GFP-Booster
Go to Chromobody Products Chromobodies
Published Scientific Literature
For a full list of publications and references which cite the use of ChromoTek products, please click here. (Note: This link will take you to the ChromoTek website)
Literature and Technical Requests
Download GFP-Trap Product Brochure Download RFP-Trap Product Brochure
Download ChromoTek
"GFP-Trap Product Brochure":

(Click on image to begin download - 1.82MB)
Download ChromoTek
"RFP-Trap Product Brochure"
:
(Click on image to begin download - 1.18MB)
   
Download GFP and RFP Booster Product Brochure  Download ChromoTek GST-Trap Product Brochure
Download ChromoTek
"GFP and RFP Booster Product Brochure"
:
(Click on image to begin download - 1.62MB)
Download ChromoTek
"GST-Trap Product Brochure"
:
(Click on image to begin download - 587KB)
   
Download ChromoTek Dnmt1-Trap Product Brochure  
Download ChromoTek
"Dnmt1-Trap Product Brochure"
:
(Click on image to begin download - 1.48MB)
 
Technical Literature Request Request Technical Literature
For detailed information go to the ChromoTek website:
Go to ChromoTek website
www.chromotek.com
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Product/Application Areas and Prices Product Groups
GFP-Trap Products
 
Go to list of GFP-Trap Products Price List
GFP-Trap Products:
  Anti-GFP VHH coupled to agarose beads: GFP-Trap®_A, GFP-Trap®_A Kit
  Anti-GFP VHH coupled to magnetic microparticles:  GFP-Trap®_M, GFP-Trap®_M Kit
  Anti-GFP VHH coupled to magnetic agarose beads: GFP-Trap®_MA, GFP-Trap®_MA Kit
  Anti-GFP VHH immobilized in 96 well Format: GFP-multiTrap®
  Binding Protein: anti-GFP VHH purified protein (GFP-Trap®, uncoupled protein)
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RFP-Trap Products
 
Go to list of RFP-Trap Products Price List
RFP-Trap Products:
  Anti-RFP VHH coupled to agarose beads: RFP-Trap®_A, RFP-Trap®_A Kit
  Anti-RFP VHH coupled to magnetic microparticles: RFP-Trap®_M, RFP-Trap®_M Kit
  Anti-RFP VHH coupled to magnetic agarose beads: RFP-Trap®_MA, RFP-Trap®_MA Kit
  Binding Protein: anti-RFP VHH purified protein (RFP-Trap®, uncoupled protein)
GST-Trap Products
 
Go to list of GST-Trap Products Price List
GST-Trap Products:
  Anti-GST VHH coupled to agarose beads: GST-Trap_A, GST-Trap_A Kit
  anti-GST VHH coupled to magnetic microparticles: GST-Trap_M, GST-Trap_M Kit
  Binding Protein: anti-GST VHH purified protein (GST-Trap, uncoupled protein)
  Specificity: Glutathione S-Transferase (GST) of Schistosoma japonicum
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MBP-Trap Products
 
Go to list of MBP-Trap Products Price List
MBP-Trap Products:
  Anti-MBP VHH coupled to agarose beads: MBP-Trap_A, MBP-Trap_A Kit
  Binding Protein: anti-MBP VHH purified protein (MBP-Trap, uncoupled protein
  Specificity: Maltose-binding protein (MBP) of Escherichia coli
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Myc-Trap Products
 
Go to list of Myc-Trap Products Price List
Myc-Trap Products:
  Anti-Myc VHH coupled to agarose beads: Myc-Trap®_A, Myc-Trap®_A Kit
  Anti-Myc VHH coupled to magnetic agarose beads: Myc-Trap®_MA, Myc-Trap®_MA Kit
  Purified Protein: Myc peptide sequence EQKLISEEDL (corresponding to amino acids 410-419 of the C-terminal of human c-myc)
 
Binding Protein: anti-Myc VHH purified protein (Myc-Trap, uncoupled protein
    Specificity: Anti-Myc reconises EQKLISEEDL at the N-terminus, C-terminus, or internal site of the fusion protein. The Myc-Trap® does NOT bind endogenous c-myc.
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Dnmt1-Trap Products
 
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Dnmt1-Trap Products:
  Anti-Dnmt1 VHH coupled to agarose beads: Dnmt1_A-Trap, Dnmt1_A-Trap Kit
  Binding Protein: anti-Dnmt1 VHH purified protein (Dnmt1-Trap, uncoupled protein)
  Specificity: Binds to human DNA (cytosine-5)-methyltransferase 1 (DNMT1)
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MK2-Trap Products
 
Go to list of Nano-Trap_MAPKK2-Trap Products Price List
MK2-Trap Products:
  Anti-MK2 VHH coupled to agarose beads: MK2-Trap,MK2-Trap Kit
  Binding Protein: anti-MK2 VHH purified protein (MK2-Trap, uncoupled protein)
  Specificity: Species-Reactivity: tested on human, mouse, hamster. Posttranslational Modifications: MK2-Trap recognizes unphosphorylated MK2 and Phospho-MK2 (Thr222). Specificity on Phospho-MK2 (Thr334) was not tested.
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p53-Trap Products
 
Go to list of p53 C-terminal-Trap Products Price List - p53 C-terminal
Go to list of p53 N-terminal-Trap Products Price List - p53 N-terminal
p53 C-terminal-Trap Products:
  Anti-p53 VHH coupled to agarose beads: p53 C-term-Trap_A, p53 C-term-Trap_A Kit
  Binding Protein: anti-p53 VHH purified protein (p53-Trap C-term, uncoupled protein)
  Specificity: Species-Reactivity: tested on human Epitope: aa 302-393
p53 N-terminal-Trap Products:
  Anti-p53 VHH coupled to agarose beads: p53 N-term-Trap_A, p53 N-term-Trap_A Kit
  Binding Protein: anti-p53 VHH purified protein (p53-Trap N-term, uncoupled protein)
  Specificity: Species-Reactivity: tested on human Epitope: aa 1-81
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PARP1-Trap Products
 
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PARP1-Trap Products:
  Anti-PARP1 VHH coupled to agarose beads: PARP1-Trap_A, PARP1TRap_A kit
  Binding Protein: anti-PARP1 VHH purified protein (PARP1, uncoupled protein)
  Specificity: Human PARP1, does not bind to human PARP2, 3, or 9. Epitope: within DNA binding domain of PARP1
 
Mdm4/ HdmX-Trap Products
 
Go to list of Mdm4/ HdmX-Trap Products Price List
Mdm4/ HdmX-Trap Products:
  Anti-Mdm4/ HdmX VHH coupled to agarose beads: Hdm4/ HdmX-Trap_A, Hdm4/ HdmX-Trap_A Kit
  Binding Protein: anti-Mdm4/ HdmX VHH purified protein (Mdm4/ HdmX-Trap, uncoupled protein)
  Specificity: Species-Reactivity: tested on human. Epitope: aa 1-129
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Chromobody Products
 
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Chromobody Products:
  Actin Chromobody® (GFP and RFP tagged) Plasmids
  Cell Cycle PCNA Chromobody® (RFP tagged) Plasmids
  Dmnt1 Chromobody® (GFP and RFP tagged) Plasmids
  Lamin Chromobody® (GFP tagged) Plasmids
  PARP1 Chromobody® (GFP and RFP tagged) Plasmids
  Vimentin Chromobody® (GFP tagged) Plasmids
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Nano Boosters
 
Go to list of Nano Booster Products Price List
Nano Booster Products:
  GFP-Booster Atto488: GFP binding protein coupled to fluorescent dye Atto488
  GFP-Booster Atto594: GFP binding protein coupled to fluorescent dye Atto594
  GFP-Booster Atto647N: GFP binding protein coupled to fluorescent dye Atto647N
  RFP-Booster Atto488: GFP binding protein coupled to fluorescent dye Atto488
  RFP-Booster Atto594: RFP binding protein coupled to fluorescent dye Atto594
  RFP-Booster Atto647N: RFP binding protein coupled to fluorescent dye Atto647N
  Vimentin-Booster Atto488: Vimentin binding protein coupled to fluorescent dye Atto488
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Monoclonal Antibodies
 
Go to list of Monoclonal Antibodies Products Price List
Rat Monoclonal Antibodies Products:
  Dnmt1 antibody [2E8]
  GFP antibody [3H9]
  GST antibody [6G9]
  HA Antibody [7C9]
  Myc-tag antibody [9E1]
  PCNA antibody [16D10]
  RFP antibody [5F8]
  RFP antibody [6G6]
  RNA Pol II antibodies (unphoshorylated epitope (1C7), Ser2-P (3E10), Ser5-P (3E8), Ser7-P (4E12))
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Binding Controls
 
Go to list of Binding Controls Price List
Binding Control Products:
  Blocked Agarose Beads: used as a binding control for GFP-Trap® Agarose Beads
  Blocked Magnetic Particles: used as a binding control for GFP-Trap® Magnetic Particles
    Blocked Magnetic Agarose Beads: used as a binding control for GFP-Trap® Magnetic Agarose Beads
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