| NEW DNA Polymerase |
| InBlood Polymerase |
InBlood DNA polymerase is a mixture of several proprietary thermostable polymerases designed for effective PCR of templates directly from blood, without DNA purification. InBlood DNA polymerase can effectively produce PCR fragment up to 1.5 kb in length at 1-25% (v/v) of blood in the reaction mixture. Hot Start feature provides high sensitivity of PCR tests with increased specificity and low background level.
| Applications: |
| |
• |
Found effective on human, mouse, rat, cat, dog, cow, sheep, pig and some species of birds such as hen and pidgeon |
| |
• |
Fresh or frozen blood collected with/without EDTA or Na-citrate can be used for PCR. Blood spots on
paper/swabs can also be used. |
| |
• |
Optimal concentration of human blood in the reaction mixture is 5-10%. Maximal concentration of human blood in the reaction mixture is 20-25%. |
| Features: |
| |
• |
5’-3’ polymerase activity |
| |
• |
3’-5’ exonuclase (Proof-reading) activity |
| |
• |
High specificity |
| |
• |
Hot start |
| |
• |
Resistance to inhibitors from blood |
|
|
| NEW DNA Polymerase |
| SNPase for SNP Detection |
SNPase is Taq DNA Polymerase with N-terminal deletion and prioprietary amino acids substitutions introduced into the active center of the enzyme. This modification causes dramatic increase of sensitivity of the enzyme to mismatches at 3’-end of the primer. Consequently, non-perfect annealing of the primers does not result in the formation of unspecific amplicons.
| Applications: |
| |
• |
SNP-genotyping, allele specific PCR, allele specific primer extension, mini-sequencing |
| |
• |
Highly specific PCR |
| |
• |
Multiplex PCR |
| |
• |
Highest fidelity PCR |
| |
• |
Real Time PCR with intercalating dyes (SYBR Green, Eva Green) |
| Features: |
| |
• |
High sensitivity to mismatches at 3’end of primers |
| |
• |
High fidelity of dNTPs and ddNTPs incorporation |
| |
• |
Lack of 5’- and 3’- exonuclease activity |
| |
• |
High specificity, low level of background PCR |
|
|
|
| NEW DNA Polymerase |
Encyclo PCR Kit for Long Range PCR, Difficult Templates, cDNA Library Construct
|
Encyclo PCR Kit is intended for most PCR and primer extension applications. It is especially recommended for cDNA amplification and cDNA library construction. Encyclo polymerase mix produces high yields of PCR products from a wide variety of templates and is suitable for difficult templates, long PCR from phage lambda DNA and cloning.
| Applications: |
| |
• |
Long range PCR |
| |
• |
Difficult templates |
| |
• |
cDNA library construction |
| Features: |
| |
• |
Highly processive 5'-3' DNA polymerase activity |
| |
• |
Proofreading 3'-5' exonuclease activity |
| |
• |
Hot Start PCR |
|
|
| NEW DNA Polymerase |
| Tersus PCR Kit for PCR of Complex Templates |
Tersus PCR Kit provides the highest possible fidelity and specificity of PCR due to the unique combination of genetically modified polymerases and special additives. The mix can be used for PCR of complex templates including repetitive sequences.
| Applications: |
| |
• |
High fidelity PCR with complex templates |
| Features: |
| |
• |
3'-5' proofreading exonuclease activity |
| |
• |
Lacks 5' exonuclease activity |
| |
• |
Hot Start PCR |
|
|
Literature on NEW DNA Polymerases
|
|
| Description |
Size |
File1 |
| InBlood Data Sheet and Protocol |
58KB |
 |
| SNPase Data Sheet and Protocol |
50KB |
|
| Encyclo PCR Kit Protocol |
109KB |
|
| Tersus PCR Kit Protocol |
219KB |
|
| Note 1: Click on the Adobe Acrobat icon to download the file |
|
| FREE DNA Polymerase Samples |
| FREE samples are now available: |
| |
| |
• |
DFS-Taq DNA Polymerase Sample |
|
| |
For a limited time only, you can request a free sample (see Note 1) of a DNA Polymerase.
Request your free DNA Polymerase sample by either emailing us at
This e-mail address is being protected from spambots. You need JavaScript enabled to view it
us or by contacting us via our Technical Enquiry form.
Note 1: Limited to one sample per customer and available only whilst stocks last. |
|
|
|
Products and Applications 
